Human isg15 primer
WebEvery primer pair is optimized, experimentally validated, and performance guaranteed. Home > Life Science Research > Products > PCR Amplification > PrimePCR™ PCR Primers, Assays, and Arrays > Gene: ISG15, Human . Gene: ISG15, Human Gene Symbol ... ISG15, Human Reaction: 200 x 20 µl reactions Gene-specific synthetic DNA template … WebDescription: Homo sapiens ISG15 ubiquitin-like modifier (ISG15), mRNA. RefSeq Summary (NM_005101): The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic …
Human isg15 primer
Did you know?
Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. ISG15 is induced by type I interferon (IFN) and serves many functions, acting both as an extracellular cytokine and an intracellular protein modifier. The precise functions are diverse and vary among species but include potentiation of Interferon gamma (IFN-II) production in lymp… WebAntibodies that detect ISG15 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA and Immunoprecipitation. These antibodies target ISG15 in Human, Mouse, Rat and Non-human primate samples. Our ISG15 polyclonal, monoclonal and recombinant …
WebMay 10, 2024 · ISG15 forward primer-GTGGACAAATGCGACGAACC; ISG15 reverse primer-TCGAAGGTCAGCCAGAACAG; ZFP36 forward primer-GACTGAGCTATGTCGGACCTT; ... All procedures performed in studies involving human participants were in accordance with the ethical standards of Union Hospital of Huazhong … WebThe biological effects of the ISG15 protein arise in part from its conjugation to cellular targets as a primary response to interferon-α/β induction and other markers of viral or parasitic infection. Recombinant full-length ISG15 has been produced for the first time in high yield by mutating Cys78 to stabilize the protein and by cloning in a C-terminal arginine cap to …
WebApr 9, 2024 · Respiratory diseases caused by respiratory syncytial virus (RSV) and human rhinovirus (HRV) are frequent causes of the hospitalization of children; nonetheless, RSV is responsible for the most severe and life-threatening illnesses. Viral infection triggers an inflammatory response, activating interferon (IFN)-mediated responses, including IFN … WebJul 11, 2005 · It is likely that ISG15 conjugation plays an important role in antiviral response because a human virus, influenza B virus, inhibits ISG15 conjugation. However, the …
WebAug 1, 2011 · Human ISG15 and NS1B-NTR were cloned into pMBP and pET15b expression vectors (Novagene), respectively, and expressed in Escherichia coli as …
WebThis paper shows that ISG15 directly regulates Human Cytomegalovirus replication and that its accumulation restricts productive virus growth. The results suggest that integrin … intricate changesWebJul 11, 2005 · However, the biological function of ISG15 modification remains unknown, largely because only a few human ISG15 target proteins have been identified. Here we purify ISG15-modified proteins from IFN-β-treated human (HeLa) cells by using double-affinity selection and use mass spectroscopy to identify a large number (158) of ISG15 … new mexico aviation property for saleWebPrimePCR™ SYBR® Green Assay: Isg15, Rat. Real-time PCR primer assay designed for SYBR ® Green gene expression analysis. Gene-specific PCR primers for the unbiased … intricate clayWebSep 11, 2012 · ISG15 is the first identified ubiquitin-like modifier, which can covalently conjugate to other cellular proteins to form ISGylated proteins. Free ISG15 is detected as … intricate carving toolWebAug 19, 2024 · Interferon-stimulated gene 15 (ISG15) is a member of the family of ubiquitin-like proteins. Similar to ubiquitin, conjugation of ISG15 to cellular proteins requires cascade reactions catalyzed by at least 2 enzymes, UbE1L and UbcH8. Expression of ISG15 and its conjugates is up-regulated in many cancer cells, yet the underlying mechanism of … new mexico aviationWebOur ISG15 polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit, Mouse and Armenian Hamster. These antibodies have been verified by Cell … new mexico average winter temperatureWebISG15 Human qPCR Primer Pair (NM_005101) Product Code HP208303 Skip to the end of the images gallery Skip to the beginning of the images gallery $170.00 Qty Add to Cart … intricate centre sherwood park